❝short-read cDNA,高通量,擅长研究「基因差异表达」;
❞
long-read cDNA,长读长,擅长「新异构体」发现、「新转录本」发现、「新基因融合」转录本发现等;
long-read RNA,长读长,擅长新异构体发现、新转录本发现、新基因融合转录本发现、研究「转录本修饰」信息。
三者详细比较如下
❝❞
基因差异表达(differential gene expression) mRNA差异剪接(differential splicing of mRNAs) 单细胞基因表达(single-cell gene expression) RNA蛋白互作(the translatome) RNA结构(the structurome) 转录异质性(spatialomics)
介绍各组学在转录调控中的作用机制,生信分析流程。
参考
根据mRNA、miRNA、lncRNA、circRNA之间的相互作用可进行关联分析,以深入研究转录调控机制,下图为各种编码和非编码RNA之间的相互作用机制。
关联分析差不多是这个意思❝
Gomes C P D C , Schroen B , Kuster G M , et al. Regulatory RNAs in Heart Failure[J]. Circulation, 2020, 141(4):313-328. Stark R , Grzelak M , Hadfield J . RNA sequencing: the teenage years[J]. Nature Reviews Genetics. https://en.wikipedia.org/wiki/File:Gene_structure_eukaryote_2_annotated.svg Conesa A , Madrigal P , Tarazona S , et al. A survey of best practices for RNA-seq data analysis[J]. Genome Biology, 2016, 17(1):181. Hrdlickova B , De Almeida R C , Borek Z , et al. Genetic variation in the non-coding genome: Involvement of micro-RNAs and long non-coding RNAs in disease.[J]. BBA - Molecular Basis of Disease, 2014, 1842(10):1910-1922. https://sysbiocube-abcc.ncifcrf.gov/public/sysbiocube_documents/sysbiocube_sops/miRNAseq.pdf https://www.researchgate.net/figure/Overview-of-the-wheat-miRNA-pipeline-The-procedure-is-divided-in-three-parts-producing_fig5_275360044 https://www.g3journal.org/content/9/4/1263 【科研】LncRNA的数据分析 Gao Y , Li S , Lai Z , et al. Analysis of Long Non-Coding RNA and mRNA Expression Profiling in Immature and Mature Bovine (Bos taurus) Testes[J]. Frontiers in Genetics, 2013, 10. https://www.futuremedicine.com/doi/pdf/10.2217/epi-2019-0295
联系客服