反相液相色谱法测定老鼠以及酵母中的同位素代谢物

用两种不同的液相分离（亲水性和疏水性）和质谱联用的技术，鉴定老鼠以及酵母中的同位素代谢物。设定了较佳的鉴定参数，并且对正常酵母和氮饥饿的酵母样品，进行同位素代谢组学鉴定。

反相液相色谱法（reverse-phase liquid chromatography，RPLC）是代谢组学中较常见和实用的一种LC分离方法。但是缺点是对于强极性物质保留较差。亲水相互作用液相色谱（hydrophilic interaction liquid chromatography，HILIC）是近年来色谱领域研究的热点之一。

它通过采用强极性度固定相，并且结合高比例有机相和低比例水相作为流动相。此前文章报道的RPLC和HILIC是主要集中在特征峰的数目方面，还没有文章系统比较这两种方法在代谢组学上的鉴定和分析。

系统地比较了8种不同条件的RPLC-MS和HILIC-MS方法在代谢组学鉴定上的不同，为代谢组学的鉴定提供新方法。

89924-82-3环丙烷羧酸-D4Cyclopropane-2,2,3,3-d4-carboxylicacid

1359833-48-97-溴萘-D7Naphthalene-1,2,3,4,5,6,8-d7,7-bromo

56004-61-6邻二甲苯-D10O-xylene-d10

153557-95-0环丙胺-D5Cyclopropylamine-d5

16649-49-3乙酸酐-D6Aceticanhydride-d6

126840-15-1邻苯二甲醚-D41,2-Dimethoxybenzene-3,4,5,6-d4

1093384-99-65-氯-2-氨基吡啶-3,4,6-D35-Chloro-2-pyridinamine-3,4,6-d3

352431-23-3对甲基苯胺-D9P-Toluidine-d9

152404-44-94-溴苯酚-2,3,5,6-D44-Bromophenol-2,3,5,6-d4

5-甲基吗啉-3-氨基-2-恶唑烷基酮-D53-amino-5-(4-morpholinylmethyl-d2)-2-oxazolidinone-4,4,5-d3

1246820-57-43,4-二氨基甲苯-D63,4-Diaminotoluene-d6

1020720-09-55-溴-6-硝基苯-1,2,3,4-D41-Bromo-2-nitrobenze-d4

1219798-78-3邻苯二胺-D81,2-Phenylenediamine-d8

64531-29-9苯并咪唑-4,5,6,7-D4Benzimidazole-4,5,6,7-d4

1374664-60-42-溴-4-甲基吡啶-D62-Bromo-4-methylpyridine-d6

1643543-24-12-溴-4-(叔丁基-D9)-吡啶2-Bromo-4-(tert-butyl-d9)-pyridine

1185306-03-92-溴-5-(甲基-D3)-吡啶2-Bromo-5-(methyl-d3)-pyridine

15502-50-8均四甲苯-D141,2,4,5-Tetramethylbenzene-d14

1354892-73-13,5-二甲氧基苯甲醛-D63,5-Dimethoxybenzaldehyde-d6

78832-61-82-羟基萘-D82-Naphthol-d8

123-62-6UN丙酸酐-D2Propanoic-2,2-d2anhydride

20201-29-0异丙苯-D62-Phenylpropane-1,1,1,3,3,3-d6

72182-69-5异丁醇-D62-Methyl-d3-propyl-3,3,3-d3alcohol

42983-07-3三甲基乙酸-D9Trimethyl-d9-aceticacid

52089-62-0丁二酸二乙酯-2,2,3,3-D4Diethylsuccinate-2,2,3,3-d4

1219798-53-4对乙酰氨基酚-D7N-(4-hydroxyphenyl-2,3,5,6-d4)acetamide-2,2,2-d3

284474-77-71,3-丙二醇-D61,3-Propane-d6-diol

1076-07-9苯乙酸-2,2-D2Phenylacetic-2,2-d2acid

1219802-12-6水杨酸甲酯-D4Methyl2-hydroxybenzoate-d4

42932-62-75-溴戊酸-3,3,4,4-D45-Bromopentanoic-3,3,4,4-d4acid

93951-91-83,3-二氯联苯胺D63,3'-Dichlorobenzidine-d6

1173021-85-6四咪唑-D5盐酸盐Tetramisole-d5hydrochloride

13122-33-3溴苯-4-D1Benzene-d,4-bromo-

13122-34-4氯苯-4-D11-Chlorobenzene-4-d

1158734-28-11,4-二羟甲基苯-D41,4-di(Hydroxymethyl)benzene-d4

202468-69-71,2-环氧丙烷-D61,2-Propylene-d6oxide

109930-25-8糠醇-D2Furfurylalcohol-d2

75736-47-9软脂酸-D4Palmiticacid-d4

35468-44-1溴代环戊烷-D9Cyclopentyl-d9bromide

1093380-08-5N-(甲基-D3)-哌嗪N-(Methyl-d3)-piperazine

207456-83-54-甲基-3-戊烯-2-酮-D104-Methyl-3-penten-2-one-d10

4303-49-5丙二酸二乙酯-D2Diethylmalonate-d2

65538-27-4苯乙酸-D7Phenylacetic-d7acid

35845-63-7苯乙醇-D52-Phenyl-d5-ethanol

溴丙烯-D5

1039678-66-44-碘甲苯-D74-Iodotoluene-d7

1014695-53-41-Boc-4-哌嗪-2,2,6,6-D41-Boc-4-Piperidone-2,2,6,6-d4

66966-38-9丙酮酸乙酯-D3Ethylpyruvate-3,3,3-d3

21668-90-6琥珀酸-D6Succinicacid-d6

62740-68-52-咪唑烷酮-D4Ethyleneurea-d4

45393-79-1哌嗪-D10Piperidine-d10

13221-54-0环丁酮-2,2,4,4-D4Cyclobutanone-2,2,4,4-d4

4067-80-5溴代异丙烷-2-D12-Bromopropane-2-d1

93951-77-02,4-二硝基酚D32,4-Dinitrophenol-d3

342611-04-54-甲氧基苯甲醛-D34-Methoxy-d3-benzaldehyde

68693-08-34-甲基苯胺-D74-(Methyl-d3)benzen-2,3,5,6-d4-amine

2200293-21-42-(甲基-D3)-苯硼酸2-(Methyl-d3)-phenylboronicacid

308796-47-63-苯基-2-丙烯酸-D73-Phenyl-2-propenoicacid-d7

1219798-55-64-甲氧基苯胺-D44-Methoxyaniline-d4

115871-50-6戊酸-D9Pentanoic-d9acid

1219794-54-3N-亚硝基二乙胺-D10N-Nitrosodiethylamine-d10

70237-41-1二异丙胺-D14Di-iso-Propyl-d14-amine

203578-32-9二丁胺-D18Di-n-Butyl-d18-amine

14770-51-5乙基-2,2-D2甲酸-D

96210-87-6双酚A-D16Bisphenola-d16

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