2017. J. Anim. Sci. 95(5): 2071-2079
日粮中粗蛋白和磷含量对生长猪回肠末端磷和氨基酸消化率的影响
P. C. Xue, D. Ragland and O. Adeola
本试验研究了日粮蛋白水平对生长猪回肠磷消化的影响。试验选用了18头安装回肠瘘管的生长猪（初始重44.2±3.2kg），试验为双9×3不完全拉丁方设计，9个处理组，3个七天的饲喂期试验，每个处理6个重复。9个处理日粮包括一个无氮日粮，以评估氨基酸的基础内源损失，8个玉米豆粕型日粮，为2×4因子设计，2个粗蛋白水平（6.9和13.4%）和4个表观全肠道可消化磷水平（0.09、0.16、0.24、0.32%）。通过豆粕和磷酸氢钙来调节日粮粗蛋白和可消化磷水平。通过添加石粉，使日粮中钙磷比例维持在2.5。每个试验阶段的最后两天持续收集食糜10小时。食糜经过低压冻干，检测分析，并计算回肠氮和磷的消化率。在低蛋白组（6.9%），日粮可消化磷含量为 0.09、0.16、0.24、0.32%的回肠可消化磷分别为0.71、1.16、1.64、2.03g/kg干物质。而相应的高蛋白组，回肠可消化磷分别为0.7、1.54、2.03、1.99g/kg干物质。日粮低蛋白水平可以显著降低回肠可消化磷（P＜0.05）.在低蛋白组中回肠可消化磷含量随日粮表观总可消化磷水平的提高而线性增加（P＜0.01），而在高蛋白组中存在线性（P＜0.01）和二次线性关系（P＜0.01）。在低蛋白和高蛋白日粮中，磷酸氢钙中磷回肠真消化率分别为54.4和75.6%。总之本试验结果表明回肠可消化磷可能受到日粮蛋白缺乏的限制。必须考虑到日粮磷消化率与蛋白含量相关。
Influence of dietary crude protein and phosphorus on ileal digestion of phosphorus and amino acids in growing pigs
P. C. Xue, D. Ragland and O. Adeola
A study was conducted to investigate the effect of dietary CP levels on ileal P digestion in growing pigs. A total of 18 ileal-cannulated pigs (44.2 ± 3.2 kg initial BW) were used in a duplicated 9 ×3 incomplete Latin square design, with 9 treatments and three 7-d experimental periods giving 6 replicates per treatment. The 9 treatments consisted of 1 N-free diet to estimate basal endogenous loss of AA and 8 corn–soybean meal–based diets in a 2 × 4 factorial arrangement, which included 2 CP levels (6.9 or 13.4%) and 4 apparent total tract digestible P (ATTDP) levels (0.09, 0.16, 0.24, or 0.32%). Soybean meal and monocalcium phosphate were used to adjust the CP level and ATTDP level, respectively. Limestone was included indiets to maintain a Ca:ATTD P ratio of 2.5 across treatments. Ileal digesta was continuously collected for 10 h on the last 2 d of each 7-d experimental period. The ileal digesta samples were lyophilized and analyzed to calculate ileal digested N and P. Data were analyzed using PROC MIXED of SAS (9.3) and contrasts were used to test the linear and quadratic effects of increasing levels of P within each CP level. In the low-CP group (6.9%), the ileal digested P was 0.71, 1.16, 1.64, and 2.03 g/kg DMI for diets that contained 0.09, 0.16, 0.24, and 0.32% ATTDP, respectively, and was 0.70, 1.54, 2.03, and 1.99 g/kg DMI, respectively, for their counter parts in the high-CP group (13.4%). A low dietary CP level decreased (P < 0.05)="" ileal="" digested="" p="" (g/kg="" dmi).="" the="" ileal="" digested="" p="" (g/kg="" dmi)="" linearly="" increased="" (p="">< 0.01)="" with="" increasing="" attd="" p="" levels="" in="" the="" low-cp="" group,="" but="" the="" pattern="" was="" linear="" (p=""><0.01) and="" quadratic="" (p="">< 0.01)="" in="" the="" high-cp="" group.="" in="" the="" low-="" and="" high-cp="" diets,="" the="" determined="" true="" ileal="" digestibility="" of="" p="" in="" monocalcium="" phosphate="" was="" 54.4="" and="" 75.6%,="" respectively.="" in="" conclusion,="" this="" research="" indicated="" that="" the="" ileal="" digestion="" of="" p="" could="" be="" limited="" by="" protein="" deficiency.="" the="" dietary="" cp="" level="" should="" be="" considered="" in="" p="" digestibility–related="">
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