七氟烷可诱发幼鼠神经激活和行为亢进

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七氟烷可诱发幼鼠神经激活和行为亢进

翻译：冯玉蓉  编辑：冯玉蓉  审校：曹莹

七氟烷是一种常用的麻醉剂，可能会引起病人躁动。然而，这种临床现象的发生机制很大程度上仍是未知的。因此，我们评估了七氟烷对小鼠神经元激活和行为的影响。10日龄小鼠吸入2%七氟烷、1%异氟烷或6%地氟烷10分钟。在小鼠失去翻正反射后1分钟，即麻醉后约2分钟，记录并评估小鼠的行为活动。麻醉1分钟后通过c-Fos表达和钙显像评估小鼠神经元的激活。丙泊酚可减少神经元的激活，被用来测量七氟烷的因果关系效应。我们发现小鼠失去翻正反射后1分钟，七氟醚可引起幼龄小鼠初级躯体感觉皮质层神经元激活增加和小鼠行为亢进。地氟醚不会引起小鼠行为亢进，异氟醚只引起边缘性的行为亢进。最后，异丙酚减弱了七氟醚诱导的幼龄小鼠神经元激活和行为亢进的增加。出人意料地，这些结果表明七氟醚诱发了幼龄小鼠的神经元激活和行为亢进。这些发现提示了七氟醚诱发患者躁动的潜在机制，并将促进未来的研究，以进一步确定麻醉药是否可通过增加神经元激活，从而诱发行为亢进。

原始文献来源：Yang L, Ton H, Zhao R, et al. Sevoflurane induces neuronal activation and behavioral hyperactivity in young mice. [J]. Sci Rep. 2020 Jul 8;10(1):11226. doi: 10.1038/s41598-020-66959-x.

Sevoflurane induces neuronal activation and behavioral hyperactivity in young mice

Abstract

sevoflurane, a commonly used anesthetic, may cause agitation in patients. However, the mechanism underlying this clinical observation remains largely unknown. We thus assessed the efects of sevoflurane on neuronal activation and behaviors in mice. Ten-day-old mice received 2% sevoflurane, 1% isoflurane, or 6% desflurane for 10minutes. The behavioral activities were recorded and evaluated at one minute afterthe loss of righting reflex in the mice, which was about two minutes after the anesthetic administration. The neuronal activation was evaluated by c-Fos expression and calcium imaging at one minute after the anesthetic administration. Propofol, which reduces neuronal activation, was used to determine the cause-and-effect of sevoflurane. We found that sevoflurane caused an increase in neuronal activation in primary somatosensory cortex of young mice and behavioral hyperactivity in the mice at one minute after the loss of righting reflex. Desflurane did not induce behavioral hyperactivity and isoflurane only caused behavioral hyperactivity with borderline signifcance. Finally, propofol attenuated the sevoflurane-induced increase in neuronal activation and behavioral hyperactivity in young mice. These results demonstrate an unexpected sevoflurane-induced increase in neuronal activation and behavioral hyperactivity in young mice. These findings suggest the potential mechanisms underlying the sevoflurane-induced agitation and will promote future studies to further determine whether anesthetics can induce behavioral hyperactivity via increasing neuronal activation.

          贵州医科大学高鸿教授课题组

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